Applications

Bitplane is dedicated to build products, which are at the cutting-edge of the imaging world and are perfectly adapted to your needs and applications. In a progressive field such as scientific imaging, Bitplane offers scientific imaging solutions for a range of applications.

Whether you need to approach an imaging need from the standpoint of a specific application, research area, or product, you'll find the resources here. The Applications web section allows you to browse various showcases and research papers to see how Bitplane products can serve your research.

Image-Based Modeling Reveals Dynamic Redistribution of DNA Damage into Nuclear Sub-Domains Costes SV, Ponomarev A, Chen JL, Nguyen D, Cucinotta FA, et al.

DNA damages are daily cellular events. If such events are left unchecked in an organism, they can lead to DNA mutations and possibly cancer over a long period of time. Consequently, cells have very efficient DNA repair machinery. Many studies have focused on the different molecular factors involved in the repair machinery, neglecting to consider the spatial context where damage occurs. Therefore, little is known about the role the nuclear architecture might have in the DNA damage response. In this study, we introduce computer modeling and image analysis tools in order to relate the position of DNA damage markers to morphologically distinct regions of the nucleus. Using these tools, we show that radiation-induced damages locate preferentially in non-condensed DNA regions or at the boundary of regions with condensed DNA. These results contradict the current dogma that the molecular response to randomly generated DNA damages is independent of their nuclear locations. Instead, this suggests the existence of repair centers in the nucleus. Overall, our approach shows that nuclear architecture plays a role in the DNA damage response, reminding us that the nucleus is not simply a soup of DNA and proteins.

Target-Induced Transcriptional Control of Dendritic Patterning
and Connectivity in Motor Neurons by the ETS Gene

Eline Vrieseling and Silvia Arber
Neuronal subpopulations have long been distinguished on the basis of dendritic morphology, but mechanisms involved in the elaboration of distinct dendrite patterns in vertebrates have remained unclear. In this issue of Cell, Vrieseling and Arber show that different motor neuron pools in the mouse spinal cord elaborate distinct dendritic trees, correlating with distinct sensory-motor connectivity. They provide evidence that the target-regulated ETS transcription factor Pea3 plays a key role in the elaboration of selective dendrite patterns and sensory-motor connectivity. The cover depicts an artificial overlay of a retrogradely labeled motor neuron pool (turquoise) and sensory afferents (purple) projecting in a transverse spinal cord section.

The Triploid Endosperm Genome of Arabidopsis Adopts a Peculiar, Parental-Dosage-Dependent Chromatin Organization

Célia Baroux, Ales Pecinka, Jörg Fuchs, Ingo Schubert and Ueli Grossniklaus
The endosperm of flowering plants is a triploid nutritious seed tissue surrounding the embryo. Baroux et al. found an atypical chromatin organization in endosperm nuclei, which responds to imbalanced parental dosage. The main image shows a three-dimensional reconstruction of a young Arabidopsis seed stained in whole mount for DNA. The endosperm nuclei (yellow) fill the seed inside the sporophytic seed coat (green), while the zygote has just divided once (two nuclei; orange). The insets from left to right show various aspects of endosperm nuclei: they appear very large compared with sporophytic nuclei and are surrounded by mitochondria, their chromatin shows atypical interspersed heterochromatin foci, their euchromatin is enriched in the heterochromatic H3K9me1 mark (green), and their chromosome territories are expanded (red). The image was created with Imaris 5.7 (Bitplane).

Collaborations

Learn more about Bitplane's extensive collaboration with some of the premier universities and research facilities in the world. >>>

Testimonial

Dr. Eric Scarfone, Karolinska Institutet, Stockholm
Imaris is the best visualization tool for extremely large data sets. >>>

Show Cases

4D-Tracking with Imaris
Dr. T. Worbs, Prof. R. Förster and, Hannover Medical School, Germany
Automated tracking of T-lymphocyte migration: The present study demonstrates that chemokine receptor CCR7 signaling enhances the motility of CD4+ T cells. >>>

High-Resolution Imaging in Zebrafish
Prof. R. Klemke and colleagues, University of California at San Diego
Metastasis, the major cause of death in cancer patients, is a highly dynamic process that occurs in multiple steps, which include disruption of cell-cell adhesion, migration of cells away from the primary tumor and intravasation into the vasculature. >>>

Biofilm: 3D Visualization
Prof. Folkesson and Prof. Molin and colleagues, BioCentrum - Technical University of Denmark
Natural conjugative plasmids express factors that can stimulate planktonicbacteria to form biofilm communities. >>>

References

The publication list contains peer reviewed publications between 1990 and 2008 that are using Imaris in one of its configurations. Browse all references using Imaris.

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Applications

Image-Based Modeling Reveals Dynamic Redistribution of DNA Damage into Nuclear Sub-Domains Costes SV, Ponomarev A, Chen JL, Nguyen D, Cucinotta FA, et al.

Target-Induced Transcriptional Control of Dendritic Patterning
and Connectivity in Motor Neurons by the ETS Gene